Cisplatin zebrafish

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August undefined, 2024

WebFeb 15, 2024 · The cisplatin was used to produce a loss of CHCs to create a murine model of deafness. The AAV vectors were delivered into the scala media through the lateral wall. Compared with the control mice, the cisplatin-treated mice exhibited significantly enhanced apoptosis and autophagy in the cochleae, accompanied by a notably decreased Pou4f3 … WebNov 1, 2007 · Five dpf zebrafish larvae, prelabeled with FM1-43FX were exposed to cisplatin at concentrations of 0, 0.25, 0.5, 0.75, 1, and 1.5 mM for 4 h. Doses were chosen empirically in pilot studies by identifying high doses of cisplatin that caused hair cell death after short durations without mortality of the zebrafish.

Cisplatin-induced hair cell loss in zebrafish (Danio rerio) lateral ...

WebDec 1, 2024 · Zebrafish may offer key insights to cisplatin ototoxicity because their lateral-line organ contains hair cells that are remarkably similar to those within the cochlea but … WebFeb 6, 2014 · Zebrafish were exposed to 1000 μM cisplatin for 6 h. We, then, periodically monitored swimming depth, total swimming distance, peak swimming velocity, and mean swimming velocity of the fish for approximately 21 days. crystal gilliland

Acute Kidney Injury Model Induced by Cisplatin in Adult …

WebMar 3, 2013 · Zebrafish were pretreated with cimetidine for 1 h, followed by cotreatment with 50 μ m cisplatin for an additional 24 h. We found no significant protection against cisplatin-induced hair cell loss for all concentrations of cimetidine evaluated ( p > 0.05, one-way ANOVA; Fig. 1a ). Figure 1. WebInner ear sensory hair cells die following exposure to aminoglycoside antibiotics or chemotherapeutics like cisplatin, leading to permanent auditory and/or balance deficits in humans. Zebrafish (Danio rerio) are used to study drug-induced sensory hair cell death since their hair cells are similar in structure and function to those found in humans. WebJul 3, 2024 · Cisplatin is a widely used chemotherapeutic agent with a high degree of ototoxicity and an average incidence of over 60% ( Karasawa and Steyger, 2015 ). Ototoxicity depends on several factors, including patient … crystal gilliland clowdus top stories

Caspase 3/GSDME-dependent pyroptosis contributes to ... - Nature

Evaluation of Cisplatin-Induced Pathology in the Larval …

WebOct 21, 2024 · The zebrafish larvae model offers two significant advantages: (i) it can be used during a screening of novel substances and compounds against cisplatin-induced hair cell damage; (ii) it can promote a better understanding of the mechanisms related to cisplatin-induced hearing loss. 21 However, studies using zebrafish are not free of … WebMay 1, 2005 · Cisplatin causes changes in the zebrafish pronephric tubule typical of those seen in mammalian kidneys. After injection of cisplatin (4.6 nl, concentration 1.5 mg/ml) at 48 hpf, zebrafish with mild ( B ) or severe ( C ) decreases in inulin clearance can be distinguished by the level of residual fluorescence at 96 hpf, 24 h after the injection ... crystal gilbert mdWebZebrafish may offer key insights to cisplatin ototoxicity because their lateral-line organ contains hair cells that are remarkably similar to those within the cochlea but are optically accessible, permitting observation of cisplatin injury in live intact hair cells. crystal gilbert ohio

"WebNov 18, 2024 · Cisplatin is an effective anticancer agent, but also causes permanent hearing loss by damaging hair cells-the sensory receptors essential for hearing. There is an urgent clinical need to protect cochlear hair cells in … " - Cisplatin zebrafish

Cisplatin zebrafish

Zebrafish embryos as an in vivo model to investigate cisplatin …

WebJul 7, 2024 · Using cisplatin to model CICI (), we performed RNA sequencing in the mouse hippocampus to identify novel molecular contributors and found profound up-regulation of the adenosine A 2A receptor (A 2A R) by cisplatin (Fig. 1A), potentially representing a mechanism for cisplatin-induced neurotoxicity.Adenosine maintains central nervous … WebJul 28, 2024 · Cisplatin is a highly effective anticancer agent that primarily functions through covalent-binding to purine bases, resulting in DNA …

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WebFeb 8, 2024 · Adult zebrafish injected with cisplatin shows decreased survival, kidney cell death, and increased inflammation markers after 24 h post-injection (hpi). In this model, … WebMar 27, 2024 · Cisplatin is a platinum-based chemotherapy compound effective against a variety of cancers. However, it can cause increased reactive oxygen species (ROS) production in auditory and vestibular tissue leading to permanent hearing and balance loss. The amino acid, L-serine, has been shown to reduce ROS in some tissue types.

WebCisplatin-induced hair cell loss in zebrafish (Danio rerio) lateral line We have used time-lapse imaging to study cisplatin-induced hair cell death in lateral line neuromasts of zebrafish larvae in vivo. We found that cisplatin-induced hair cell death occurred much more slowly than had been shown to occur in aminoglycoside-induced hair cell death. WebDec 1, 2024 · Cisplatin ototoxicity Zebrafish Mitochondrial dysfunction Mitochondrial bioenergetics 1. Introduction Cisplatin is a chemotherapeutic agent that is commonly used in the treatment of solid tumors.

WebConclusion: The zebrafish lateral line was used to identify 2 small molecules that protected against cisplatin-induced hair cell death. Key Words: CisplatinVHair cellVOtotoxicityV Protection. Otol Neurotol 36:519Y525, 2015. Cisplatin is a commonly used anticancer drug in the treatmentofmanycancers,includinglungcancer,ovarian WebUsing zebrafish embryos as a model, cisplatin was previously found to impair skin ionocytes and ion regulation. The purpose of this study was to further investigate how cisplatin damages ionocytes. Zebrafish embryos were exposed to cisplatin (0, 50, and 100 μM) for 96 h (4-100 h post-fertilization) and then stained with fluorescent dyes to ...

WebMay 15, 2024 · The zebrafish has appeared as an interesting model to study renal function, kidney regeneration, and injury, as renal structures conserve functional similarities with mammals. Adult zebrafish injected with cisplatin shows decreased survival, kidney cell death, and increased inflammation markers after 24 h post-injection (hpi).

WebOur findings demonstrate that cisplatin can alter the locomotion behavior of zebrafish. Behavioral changes of zebrafish according to cisplatin-induced toxicity of the balance … crystal giles ameripriseWebFeb 18, 2024 · For this study, adult female zebrafish were given cisplatin (G2), the probiotic, Bacillus megaterium (G3), and cisplatin+ B. megaterium (G4) for 30 days, in addition to the control (G1). In order to investigate changes in antioxidative enzymes, ROS production, and histological changes after treatment, the intestines and ovaries were … dwelling coverage vs homeowners insuranceWebJan 1, 2024 · Exposure of zebrafish larvae (5dpf) to 1000 μM of cisplatin for 4 h induced extensive loss of hair cells in the neuromasts in the anterior, middle, and posterior regions ( Fig. 1 ). A similar loss of hair cells with a dose of cisplatin ranging from 250 to 1500 μM cisplatin was reported by Ou et al. (2007). dwelling coverage replacement costWebFeb 15, 2024 · Cisplatin or doxorubicin induces pyroptosis of renal tubular epithelial cells It has been reported that the typical characteristics of pyroptosis were increased LDH release, increased PI-positive... dwelling definition orsWebJan 1, 2024 · This study provides evidence that cisplatin-induced hair cell loss in zebrafish is accompanied by protein nitration and Lmo4 degradation, which are emerging as … crystal gillery crystal gillsWebMar 6, 2013 · Cisplatin, one of the most commonly used anticancer drugs, is known to cause inner ear hair cell damage and hearing loss. Despite much investigation into mechanisms of cisplatin-induced hair cell death, little is known about the mechanism whereby cisplatin is selectively toxic to hair cells. crystal gillis np